FABP4 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant FABP4.
Immunogen
Recombinant protein corresponding to human FABP4.
Sequence
DAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYE
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (Human: 1:100-1:250, Mouse/Rat: 1:100-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of RT-4 cell lysates using FABP4 polyclonal antibody (Cat # PAB28577).Western Blot (Cell lysate)
Western blot analysis of lane 1: NIH-3T3 and lane 2: NBT-II cell lysates using FABP4 polyclonal antibody (Cat # PAB28577).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human soft tissue with FABP4 polyclonal antibody (Cat # PAB28577) shows distinct positivity in adipocytes. Retrieval method: HIER pH6Immunofluorescence
Immunofluorescence of A-431 cell line with FABP4 polyclonal antibody (Cat # PAB28577) shows positivity in cytoplasm. Fixation/Permeabilization: PFA/Triton X-105 -
Gene Info — FABP4
Entrez GeneID
2167Protein Accession#
P15090Gene Name
FABP4
Gene Alias
A-FABP, aP2
Gene Description
fatty acid binding protein 4, adipocyte
Omim ID
600434Gene Ontology
HyperlinkGene Summary
FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq
Other Designations
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