CYB5R3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with CYB5R3 polyclonal antibody (Cat # PAB28452) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with CYB5R3 polyclonal antibody (Cat # PAB28452) shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells at 1:1000-1:2500 dilution.
Immunofluorescence
Immunofluorescent staining of U373 MG with CYB5R3 polyclonal antibody (Cat # PAB28452) at 1-4 ug/mL dilution shows positivity in endoplasmic reticulum.
-
Specification
Product Description
Rabbit polyclonal antibody raised against recombinant CYB5R3.
Immunogen
Recombinant protein corresponding to amino acids of recombinant CYB5R3.
Sequence
SGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDH
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry(1:1000-1:2500)
Western Blot(1:100-1:250)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with CYB5R3 polyclonal antibody (Cat # PAB28452) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with CYB5R3 polyclonal antibody (Cat # PAB28452) shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells at 1:1000-1:2500 dilution.Immunofluorescence
Immunofluorescent staining of U373 MG with CYB5R3 polyclonal antibody (Cat # PAB28452) at 1-4 ug/mL dilution shows positivity in endoplasmic reticulum. -
Gene Info — CYB5R3
Entrez GeneID
1727Protein Accession#
P00387Gene Name
CYB5R3
Gene Alias
B5R, DIA1
Gene Description
cytochrome b5 reductase 3
Omim ID
250800Gene Ontology
HyperlinkGene Summary
This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq
Other Designations
NADH-cytochrome b5 reductase|OTTHUMP00000028761|cytochrome b5 reductase|diaphorase (NADH) (cytochrome b-5 reductase)
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com