COL2A1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of COL2A1.
Immunogen
A synthetic peptide corresponding to human COL2A1.
Host
Rabbit
Theoretical MW (kDa)
140
Reactivity
Human, Mouse, Rat
Specificity
COL2A1 polyclonal antibody detects endogenous levels of COL2A1 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of COL2A1 polyclonal antibody (Cat # PAB27039) in extracts from COLO 205 cell.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of COL2A1 polyclonal antibody (Cat # PAB27039) in paraffin-embedded human breast carcinoma tissue.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — COL2A1
Entrez GeneID
1280Protein Accession#
P02458Gene Name
COL2A1
Gene Alias
ANFH, AOM, COL11A3, MGC131516, SEDC
Gene Description
collagen, type II, alpha 1
Gene Ontology
HyperlinkGene Summary
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000195063|cartilage collagen|chondrocalcin|collagen II, alpha-1 polypeptide
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Interactome
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Disease
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