CCNB1 (phospho S147) polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CCNB1 (phospho S147) polyclonal antibody (Cat # PAB25912).
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using CCNB1 (phospho S147) polyclonal antibody (Cat # PAB25912, red).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CCNB1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S147 of human CCNB1.
Sequence
A-F-SP-D-V
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CCNB1 (phospho S147) polyclonal antibody (Cat # PAB25912).Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using CCNB1 (phospho S147) polyclonal antibody (Cat # PAB25912, red). -
Gene Info — CCNB1
Entrez GeneID
891Protein Accession#
P14635Gene Name
CCNB1
Gene Alias
CCNB
Gene Description
cyclin B1
Omim ID
123836Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq
Other Designations
G2/mitotic-specific cyclin B1
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Interactome
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Disease
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