SMAD2 (phospho T220) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with IL-4 (100 ug/mL, 30 min) using SMAD2 (phospho T220) polyclonal antibody (Cat # PAB25890).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T220 of human SMAD2.
Sequence
P-E-Tp-P-P
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with IL-4 (100 ug/mL, 30 min) using SMAD2 (phospho T220) polyclonal antibody (Cat # PAB25890). -
Gene Info — SMAD2
Entrez GeneID
4087Protein Accession#
Q15796Gene Name
SMAD2
Gene Alias
JV18, JV18-1, MADH2, MADR2, MGC22139, MGC34440, hMAD-2, hSMAD2
Gene Description
SMAD family member 2
Omim ID
601366Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
MAD, mothers against decapentaplegic homolog 2|Mad protein homolog|Mad, mothers against decapentaplegic homolog 2|Mad-related protein 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2
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Interactome
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Pathway
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Disease
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