KRT8 (phospho S74) polyclonal antibody
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Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using KRT8 (phospho S74) polyclonal antibody (Cat # PAB25879, red).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of KRT8.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S74 of human KRT8.
Sequence
L-L-Sp-P-L
Host
Rabbit
Theoretical MW (kDa)
55
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using KRT8 (phospho S74) polyclonal antibody (Cat # PAB25879, red). -
Gene Info — KRT8
Entrez GeneID
3856Protein Accession#
P05787Gene Name
KRT8
Gene Alias
CARD2, CK8, CYK8, K2C8, K8, KO
Gene Description
keratin 8
Gene Ontology
HyperlinkGene Summary
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
Other Designations
cytokeratin 8|keratin, type II cytoskeletal 8
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Interactome
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Disease
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