SRC (phospho Y529) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells using SRC (phospho Y529) polyclonal antibody (Cat # PAB25415).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SRC (phospho Y529) polyclonal antibody (Cat # PAB25415).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of SRC.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y529 of human SRC.
Sequence
P-Q-Yp-Q-P
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells using SRC (phospho Y529) polyclonal antibody (Cat # PAB25415).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SRC (phospho Y529) polyclonal antibody (Cat # PAB25415). -
Gene Info — SRC
Entrez GeneID
6714Protein Accession#
P12931Gene Name
SRC
Gene Alias
ASV, SRC1, c-SRC, p60-Src
Gene Description
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Omim ID
190090Gene Ontology
HyperlinkGene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174476|OTTHUMP00000174477|proto-oncogene tyrosine-protein kinase SRC|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1
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Interactome
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Pathway
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Disease
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