EIF4EBP1 (phospho T45) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200 nM, 5 min), using EIF4EBP1 (phospho T45) polyclonal antibody (Cat # PAB25350).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EIF4EBP1 (phospho T45) polyclonal antibody (Cat # PAB25350).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of EIF4EBP1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T45 of human EIF4EBP1.
Sequence
S-T-Tp-P-G
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200 nM, 5 min), using EIF4EBP1 (phospho T45) polyclonal antibody (Cat # PAB25350).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EIF4EBP1 (phospho T45) polyclonal antibody (Cat # PAB25350). -
Gene Info — EIF4EBP1
Entrez GeneID
1978Protein Accession#
Q13541Gene Name
EIF4EBP1
Gene Alias
4E-BP1, 4EBP1, BP-1, MGC4316, PHAS-I
Gene Description
eukaryotic translation initiation factor 4E binding protein 1
Omim ID
602223Gene Ontology
HyperlinkGene Summary
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq
Other Designations
eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
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Interactome
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Pathway
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Disease
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