ACTL6B polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with ACTL6B polyclonal antibody (Cat # PAB24389) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with ACTL6B polyclonal antibody (Cat # PAB24389) shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts at 1:200-1:500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with ACTL6B polyclonal antibody (Cat # PAB24389) at 1-4 ug/mL dilution shows positivity in nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ACTL6B.
Immunogen
Recombinant protein corresponding to amino acids of human ACTL6B.
Sequence
CPKADFPTTVGLLAAEEGGGLELEGDKEKKGKIFHIDTNALHV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with ACTL6B polyclonal antibody (Cat # PAB24389) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with ACTL6B polyclonal antibody (Cat # PAB24389) shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with ACTL6B polyclonal antibody (Cat # PAB24389) at 1-4 ug/mL dilution shows positivity in nucleoli. -
Gene Info — ACTL6B
Entrez GeneID
51412Protein Accession#
O94805Gene Name
ACTL6B
Gene Alias
ACTL6, BAF53B
Gene Description
actin-like 6B
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. [provided by RefSeq
Other Designations
53 kDa BRG1-associated factor B|actin-like 6|actin-related protein|hArpN alpha
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Interactome
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Disease
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