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Last updated: 2014/8/25
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INO80B polyclonal antibody

  • Catalog # : PAB23972
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant INO80B.
  • Immunogen:
  • Recombinant protein corresponding to amino acids of human INO80B.
  • Sequence:
  • PMVRYCSGAQGSTLSFPPGVPAPTAVSQRPSPSGPPPRCSVPGCPHPRRYACSRTGQALCSLQCYRINLQMRLGGPEGPGS
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:200)
    Immunofluorescence (1-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.5 (40% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
    Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with INO80B polyclonal antibody (Cat # PAB23972).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human kidney with INO80B polyclonal antibody (Cat # PAB23972) shows strong cytoplasmic and nuclear positivity in cells in tubules at 1:50-1:200 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of human cell line A-431 with INO80B polyclonal antibody (Cat # PAB23972) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • INO80B
  • Gene Alias:
  • HMGA1L4,HMGIYL4,IES2,PAP-1BP,PAPA-1,ZNHIT4,hIes2
  • Gene Description:
  • INO80 complex subunit B
  • Other Designations:
  • IES2 homolog,OTTHUMP00000160419,PAP (Pim-1 associated protein) associated protein 1,PAP-1 binding protein,high mobility group AT-hook 1-like 4,zinc finger, HIT type 4
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