Immunohistochemistry (1:50-1:200) Western Blot (1:250-1:500) Immunofluorescence (1-4 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.5 (40% glycerol, 0.02% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with IGFALS polyclonal antibody (Cat # PAB23681) at 1:250-1:500 dilution.
Immunohistochemical staining of human testis with IGFALS polyclonal antibody (Cat # PAB23681) shows moderate cytoplasmic positivity in cells in seminiferus ducts at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with IGFALS polyclonal antibody (Cat # PAB23681) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.
The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations:
INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN COMPLEX ACID LABILE CHAIN