SPAG16 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant SPAG16.
Immunogen
Recombinant protein corresponding to amino acids of human SPAG16.
Sequence
NLKKDLKHYKQAADKAREDLLKIQKERDFHRMHHKRIVQEKNKLINDLKGLKLHYASYEPTIRVLHEKHHTLLKEKMLTSLERDKVVG
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with SPAG16 polyclonal antibody (Cat # PAB23127) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast with SPAG16 polyclonal antibody (Cat # PAB23127) shows strong cytoplasmic positivity in glandular cells at 1:200-1:500 dilution. -
Gene Info — SPAG16
Entrez GeneID
79582Protein Accession#
Q8N0X2Gene Name
SPAG16
Gene Alias
DKFZp666P1710, FLJ22724, FLJ37717, MGC87036, PF20, WDR29
Gene Description
sperm associated antigen 16
Gene Ontology
HyperlinkGene Summary
Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM
Other Designations
WD repeat domain 29|sperm-associated WD repeat protein
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Interactome
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Disease
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