Rabbit polyclonal antibody raised against synthetic peptide of FGFR1.
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human FGFR1.
Protein G purification
ELISA (1:1000) Western Blot (1:100-500) Immunohistochemistry (1:50-100) Immunoprecipitation (1:50-100) Immunofluorescence (1:50-100) Flow cytometry (1:10-50) The optimal working dilution should be determined by the end user.
In PBS (0.09% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with FGFR1 polyclonal antibody (Cat # PAB2083) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF-2 in the presence or absence of 10 ng/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 10 ng/mL FGFR1 and 7E3 antibody. FGFR is visualized as red fluorescence (i, iv, vii), A1B3 is visualized as green fluorescence (ii, v, viii), and colocalization of FGF-2 and fibrinogen receptors is shown as yellow fluorescence (iii, vi, ix). Insets represent the background staining for red (i) and green (ii) fluorescence. Bars represent 25 .
Flow cytometric analysis of MCF-7 cells using FGFR1 polyclonal antibody (Cat # PAB2083)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq