DCK polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The DCK polyclonal antibody (Cat # PAB2020) is used in Western blot to detect DCK in mouse intestine tissue lysate.
Immunoprecipitation
Deoxycytidine kinase (DCK) immunoprecipitated from NCI-H460 cells with DCK polyclonal antibody (Cat # PAB2020) using the Pierce classic mammalian IP kit reagent as described as manufacturer instructions (lane 1, 3) and current protocols in Cell Biology, 1998, 7.2.1-7.2.21.
Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with DCK polyclonal antibody (Cat # PAB2020).
Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit).
No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DCK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DCK.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunoprecipitation (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The DCK polyclonal antibody (Cat # PAB2020) is used in Western blot to detect DCK in mouse intestine tissue lysate.Immunoprecipitation
Deoxycytidine kinase (DCK) immunoprecipitated from NCI-H460 cells with DCK polyclonal antibody (Cat # PAB2020) using the Pierce classic mammalian IP kit reagent as described as manufacturer instructions (lane 1, 3) and current protocols in Cell Biology, 1998, 7.2.1-7.2.21.
Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with DCK polyclonal antibody (Cat # PAB2020).
Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit).
No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago. -
Gene Info — DCK
Entrez GeneID
1633Protein Accession#
NP_000779;P27707Gene Name
DCK
Gene Alias
MGC117410, MGC138632
Gene Description
deoxycytidine kinase
Omim ID
125450Gene Ontology
HyperlinkGene Summary
Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
- Acute Disease
- Adenocarcinoma
- Breast cancer
- Breast Neoplasms
- Carcinoma
- Kidney Failure
- Leukemia
- Neoplasms
- Neutropenia
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Publication Reference
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Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity.
Hartford CM, Duan S, Delaney SM, Mi S, Kistner EO, Lamba JK, Huang RS, Dolan ME.
Blood 2008 Dec; 113(10):2145.
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Pharmacogenetics of deoxycytidine kinase: identification and characterization of novel genetic variants.
Lamba JK, Crews K, Pounds S, Schuetz EG, Gresham J, Gandhi V, Plunkett W, Rubnitz J, Ribeiro R.
The Journal of Pharmacology and Experimental Therapeutics 2007 Sep; 323(3):935.
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Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity.
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