FXN polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of FXN polyclonal antibody (Cat # PAB2010) in 293 cell line lysates (35 ug/lane). FXN (arrow) was detected using the purified Polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of FXN (arrow) using FXN polyclonal antibody (Cat # PAB2010). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FXN gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FXN polyclonal antibody (Cat # PAB2010) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FXN.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human FXN.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of FXN polyclonal antibody (Cat # PAB2010) in 293 cell line lysates (35 ug/lane). FXN (arrow) was detected using the purified Polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of FXN (arrow) using FXN polyclonal antibody (Cat # PAB2010). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FXN gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FXN polyclonal antibody (Cat # PAB2010) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — FXN
Entrez GeneID
2395Protein Accession#
NP_000135;Q16595Gene Name
FXN
Gene Alias
CyaY, FA, FARR, FRDA, MGC57199, X25
Gene Description
frataxin
Gene Ontology
HyperlinkGene Summary
This nuclear gene encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000021428
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Interactome
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Disease
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