Sheep polyclonal antibody raised against synthetic phosphopeptide of ATM.
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding S1981 of human ATM.
Host:
Sheep
Reactivity:
Human
Specificity:
This polyclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human ATM and cross reactivity with ATM from other mammalian sources has not been tested.
Form:
Liquid
Purification:
Affinity purification
Concentration:
1 mg/mL
Recommend Usage:
ELISA (1:2000-1:10000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of hydrogen peroxide stimulated human derived MCF-7 whole cell lysate with ATM (phospho S1981) polyclonal antibody (Cat # PAB19950).
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations:
AT mutated,TEL1, telomere maintenance 1, homolog,ataxia telangiectasia mutated (includes complementation groups A, C and D),ataxia telangiectasia mutated protein,human phosphatidylinositol 3-kinase homolog,serine-protein kinase ATM