NEDD1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of S phase HeLa cells with NEDD1 polyclonal antibody (Cat # PAB19928) at 1:10000 dilution. Lane 1: lysates from sh-Luciferase lentivirus-infected cells as control; Lane 2: lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant NEDD1.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 343-667 of human NEDD1.
Host
Rabbit
Theoretical MW (kDa)
73
Reactivity
Human
Specificity
This antibody reacts with endogenous Nedd1 protein. A BLAST analysis was used to suggest reactivity with Nedd1 from human, chimpanzee, macaque, marmoset, cattle, rat, and mouse based on a 100% homology with the immunizing sequence. Expect partial reactivity with Nedd1 from turkey, chicken, salmon, and Danio based on a 91% homology with the immunizing sequence. Cross-reactivity with Nedd1 from other sources has not been determined.
Form
Liquid
Purification
Immunoaffinity purification
Recommend Usage
ELISA (1:100000)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of S phase HeLa cells with NEDD1 polyclonal antibody (Cat # PAB19928) at 1:10000 dilution. Lane 1: lysates from sh-Luciferase lentivirus-infected cells as control; Lane 2: lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NEDD1
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Interactome
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