CYB5R3 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
CYB5R3 polyclonal antibody (Cat # PAB19649) (0.01 ug/mL) staining of human umbilical cord lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human adrenal gland with CYB5R3 polyclonal antibody (Cat # PAB19649) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CYB5R3.
Immunogen
A synthetic peptide corresponding to C-terminus of human CYB5R3.
Sequence
CLPNLDHVGHPTER
Host
Goat
Theoretical MW (kDa)
38.2
Reactivity
Human
Specificity
This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1).
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(3-5 ug/mL)
ELISA (1:32000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CYB5R3 polyclonal antibody (Cat # PAB19649) (0.01 ug/mL) staining of human umbilical cord lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human adrenal gland with CYB5R3 polyclonal antibody (Cat # PAB19649) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CYB5R3
Entrez GeneID
1727Protein Accession#
NP_000389.1;NP_001123291.1;NP_001165131.1Gene Name
CYB5R3
Gene Alias
B5R, DIA1
Gene Description
cytochrome b5 reductase 3
Omim ID
250800Gene Ontology
HyperlinkGene Summary
This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq
Other Designations
NADH-cytochrome b5 reductase|OTTHUMP00000028761|cytochrome b5 reductase|diaphorase (NADH) (cytochrome b-5 reductase)
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Interactome
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Pathway
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Disease
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