PIK3R1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of tissue and cell extracts with PIK3R1 polyclonal antibody (Cat # PAB19524).
Lane 1, rat skeletal muscle tissue lysate.
Lane 2, rat liver tissue lysate.
Lane 3, rat brain tissue lysate.
Lane 4, rat small intestine tissue lysate.
Lane 5, MCF-7 whole cell lysate.
Lane 6, HeLa whole cell lysate.
Lane 7, SMMC whole cell lysate.
Lane 8, Jurkat whole cell lysate.
Lane 9, COLO 320 whole cell lysate.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIK3R1.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human PIK3R1.
Host
Rabbit
Reactivity
Human, Rat
Specificity
It identical to the rat and mouse sequences.
Form
Lyophilized
Purification
Immunoaffinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of tissue and cell extracts with PIK3R1 polyclonal antibody (Cat # PAB19524).
Lane 1, rat skeletal muscle tissue lysate.
Lane 2, rat liver tissue lysate.
Lane 3, rat brain tissue lysate.
Lane 4, rat small intestine tissue lysate.
Lane 5, MCF-7 whole cell lysate.
Lane 6, HeLa whole cell lysate.
Lane 7, SMMC whole cell lysate.
Lane 8, Jurkat whole cell lysate.
Lane 9, COLO 320 whole cell lysate. -
Gene Info — PIK3R1
Entrez GeneID
5295Gene Name
PIK3R1
Gene Alias
GRB1, p85, p85-ALPHA
Gene Description
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Omim ID
171833Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)|phosphatidylinositol 3-kinase, regulatory, 1|phosphatidylinositol 3-kinase-associated p-85 alpha|phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)|phosphoinositide-3-ki
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Interactome
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Pathway
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Disease
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