ATOH1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of ATOH1 (arrow) using ATOH1 polyclonal antibody (Cat # PAB1947). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATOH1 gene (Lane 2) (Origene Technologies).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATOH1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ATOH1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of ATOH1 (arrow) using ATOH1 polyclonal antibody (Cat # PAB1947). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATOH1 gene (Lane 2) (Origene Technologies). -
Gene Info — ATOH1
Entrez GeneID
474Protein Accession#
NP_005163;Q92858Gene Name
ATOH1
Gene Alias
ATH1, HATH1, MATH-1, bHLHa14
Gene Description
atonal homolog 1 (Drosophila)
Omim ID
601461Gene Ontology
HyperlinkGene Summary
This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. [provided by RefSeq
Other Designations
atonal homolog 1
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Interactome
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Publication Reference
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Proteasomal degradation of Atoh1 by aberrant Wnt signaling maintains the undifferentiated state of colon cancer.
Aragaki M, Tsuchiya K, Okamoto R, Yoshioka S, Nakamura T, Sakamoto N, Kanai T, Watanabe M.
Biochemical and Biophysical Research Communications 2008 Apr; 368(4):923.
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Gene expression profiling identifies Hes6 as a transcriptional target of ATOH1 in cochlear hair cells.
Scheffer D, Sage C, Corey DP, Pingault V.
FEBS Letters 2007 Oct; 581(24):4651.
Application：Func, WB-Ce, Human, HeLa cells.
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Proteasomal degradation of Atoh1 by aberrant Wnt signaling maintains the undifferentiated state of colon cancer.
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