AFP polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of AFP in human liver tissue lysate with AFP polyclonal antibody (Cat # PAB19287) at 1 ug/mL.
Immunohistochemistry
Immunohistochemical staining of (A) mouse liver tissue with AFP polyclonal antibody (Cat # PAB19287) at 2.5 ug/mL (B) rat liver tissue with AFP polyclonal antibody (Cat # PAB19287) at 10 ug/mL.
Immunofluorescence
Immunofluorescence staining of rat liver with AFP polyclonal antibody (Cat # PAB19287) at 20 ug/mL.
Immunofluorescence
Immunofluorescence staining of mouse liver tissue with AFP polyclonal antibody (Cat # PAB19287) at 20 ug/mL.
Red: AFP polyclonal antibody (Cat # PAB19287) Blue: DAPI staining
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Specification
Product Description
Rabbit polyclonal antibody rasied against synthetic peptide of AFP.
Immunogen
A synthetic peptide corresponding to 14 amino acids near the center of human AFP. The immunogen is located within amino acids 340-390 of AFP.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
ELISA
Immunofluorescence (20 ug/mL)
Immunohistochemistry (10 ug/mL)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide).
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. Antibodies should not be exposed to prolonged high temperatures.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of AFP in human liver tissue lysate with AFP polyclonal antibody (Cat # PAB19287) at 1 ug/mL.Immunohistochemistry
Immunohistochemical staining of (A) mouse liver tissue with AFP polyclonal antibody (Cat # PAB19287) at 2.5 ug/mL (B) rat liver tissue with AFP polyclonal antibody (Cat # PAB19287) at 10 ug/mL.Immunofluorescence
Immunofluorescence staining of rat liver with AFP polyclonal antibody (Cat # PAB19287) at 20 ug/mL.Immunofluorescence
Immunofluorescence staining of mouse liver tissue with AFP polyclonal antibody (Cat # PAB19287) at 20 ug/mL.
Red: AFP polyclonal antibody (Cat # PAB19287) Blue: DAPI stainingEnzyme-linked Immunoabsorbent Assay
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Gene Info — AFP
Entrez GeneID
174Protein Accession#
NP_001125Gene Name
AFP
Gene Alias
FETA, HPAFP
Gene Description
alpha-fetoprotein
Omim ID
104150Gene Ontology
HyperlinkGene Summary
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq
Other Designations
OTTHUMP00000160480|alpha-1-fetoprotein|alpha-fetoglobulin
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Interactome
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Disease
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