BMPR2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of human heart lysate. Cat # PAB1907 at 1:1000 dilution + lysates/proteins at 20 ug per lane. Secondary gat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/10000 dilution. Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney tissue reacted with Cat # PAB1907, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using BMPR2 polyclonal antibody (Cat # PAB1907)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BMPR2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 28-59 at N-terminus of human BMPR2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Preparation Method
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Recommend Usage
Flow cytometry (1:10-1:50)
Immunohistochemistry (1:10-1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C for up to 2 weeks. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of human heart lysate. Cat # PAB1907 at 1:1000 dilution + lysates/proteins at 20 ug per lane. Secondary gat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/10000 dilution. Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney tissue reacted with Cat # PAB1907, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of HepG2 cells using BMPR2 polyclonal antibody (Cat # PAB1907)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — BMPR2
Entrez GeneID
659Protein Accession#
NP_001195; Q13873Gene Name
BMPR2
Gene Alias
BMPR-II, BMPR3, BMR2, BRK-3, FLJ41585, FLJ76945, PPH1, T-ALK
Gene Description
bone morphogenetic protein receptor, type II (serine/threonine kinase)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease. [provided by RefSeq
Other Designations
BMP type II receptor|bone morphogenetic protein receptor type II|bone morphogenetic protein receptor type-2|primary pulmonary hypertension 1|serine/threonine kinase|type II activin receptor-like kinase|type II receptor for bone morphogenetic protein-4
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression of gremlin, a bone morphogenetic protein antagonist,is associated with vascular calcification in uraemia.
Jara A, Chacon C, Burgos ME, Droguett A, Valdivieso A, Ortiz M, Troncoso P, Mezzano S.
Nephrology, Dialysis, Transplantation 2008 Nov; 24(4):1121.
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Dysfunction of Golgi tethers, SNAREs, and SNAPs in monocrotaline-induced pulmonary hypertension.
Sehgal PB, Mukhopadhyay S, Xu F, Patel K, Shah M.
American Journal of Physiology. Lung Cellular and Molecular Physiology 2007 Mar; 292(6):L1526.
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Expression of gremlin, a bone morphogenetic protein antagonist,is associated with vascular calcification in uraemia.
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