HMGB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lovo (Lane 1) and HeLa (Lane 2) cell lysate with HMGB1 polyclonal antibody (Cat # PAB18868) at 1 : 500 dilution.
Immunofluorescence
Immunofluorescent staining of HeLa cells showing nuclear staining with HMGB1 polyclonal antibody (Cat # PAB18868) at 1 : 100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant HMGB1.
Immunogen
Recombinant protein corresponding to full length human HMGB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.02% sodium azide
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lovo (Lane 1) and HeLa (Lane 2) cell lysate with HMGB1 polyclonal antibody (Cat # PAB18868) at 1 : 500 dilution.Immunofluorescence
Immunofluorescent staining of HeLa cells showing nuclear staining with HMGB1 polyclonal antibody (Cat # PAB18868) at 1 : 100 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HMGB1
Entrez GeneID
3146Protein Accession#
NM_002128Gene Name
HMGB1
Gene Alias
DKFZp686A04236, HMG1, HMG3, SBP-1
Gene Description
high-mobility group box 1
Omim ID
163905Gene Ontology
HyperlinkOther Designations
Amphoterin|OTTHUMP00000018199|OTTHUMP00000190860|Sulfoglucuronyl carbohydrate binding protein|high mobility group box 1|high mobility group protein 1|high-mobility group (nonhistone chromosomal) protein 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Dual peptide-dendrimer conjugate inhibits acetylation of transforming growth factor β-induced protein and improves survival in sepsis.
Wonhwa Lee, Eun Ji Park, Oh Kwang Kwon, Hyelim Kim, Youngbum Yoo, Shin-Woo Kim, Young-Kyo Seo, In-San Kim, Dong Hee Na, Jong-Sup Bae.
Biomaterials 2020 Jul; 246:120000.
Application：ELISA, Mouse, Mouse blood.
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Autophagy and mitochondrial remodelling in mouse mesenchymal stromal cells challenged with Staphylococcus epidermidis.
Gorbunov NV, McDaniel DP, Zhai M, Liao PJ, Garrison BR, Kiang JG.
Journal of Cellular and Molecular Medicine 2015 May; 19(5):1133.
Application：IF, Mouse, Bone marrow mesenchymal stromal cells (BMSCs).
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Dual peptide-dendrimer conjugate inhibits acetylation of transforming growth factor β-induced protein and improves survival in sepsis.
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