Goat polyclonal antibody raised against synthetic peptide of PRDM1.
Immunogen:
A synthetic peptide corresponding to internal region of human PRDM1.
Sequence:
C-DISDNADRLEDVED
Host:
Goat
Theoretical MW (kDa):
90
Reactivity:
Human
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Recommend Usage:
ELISA (1:8000) Western Blot (0.3-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
PRDM1 polyclonal antibody (Cat # PAB18739) (0.3 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq
Other Designations:
B-lymphocyte-induced maturation protein 1,OTTHUMP00000016918,PR-domain zinc finger protein 1,PRDI-binding factor-1,beta-interferon gene positive-regulatory domain I binding factor,positive regulatory domain I-binding factor 1