PRDM1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
PRDM1 polyclonal antibody (Cat # PAB18739) (0.3 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PRDM1 polyclonal antibody (Cat # PAB18739) (3.8 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PRDM1.
Immunogen
A synthetic peptide corresponding to internal region of human PRDM1.
Sequence
C-DISDNADRLEDVED
Host
Goat
Theoretical MW (kDa)
90
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PRDM1 polyclonal antibody (Cat # PAB18739) (0.3 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PRDM1 polyclonal antibody (Cat # PAB18739) (3.8 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRDM1
Entrez GeneID
639Protein Accession#
NP_001189.2;NP_878911.1Gene Name
PRDM1
Gene Alias
BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1
Gene Description
PR domain containing 1, with ZNF domain
Omim ID
603423Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq
Other Designations
B-lymphocyte-induced maturation protein 1|OTTHUMP00000016918|PR-domain zinc finger protein 1|PRDI-binding factor-1|beta-interferon gene positive-regulatory domain I binding factor|positive regulatory domain I-binding factor 1
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Interactome
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Disease
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