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Last updated: 2014/10/20
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PRDM1 polyclonal antibody

  • Catalog # : PAB18739
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PRDM1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human PRDM1.
  • Sequence:
  • C-DISDNADRLEDVED
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 90
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:8000)
    Western Blot (0.3-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • PRDM1 polyclonal antibody (Cat # PAB18739) (0.3 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • PRDM1 polyclonal antibody (Cat # PAB18739) (3.8 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 639
  • Protein Accession#:
  • NP_001189.2;NP_878911.1
  • Gene Name:
  • PRDM1
  • Gene Alias:
  • BLIMP1,MGC118922,MGC118923,MGC118924,MGC118925,PRDI-BF1
  • Gene Description:
  • PR domain containing 1, with ZNF domain
  • Gene Summary:
  • This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • B-lymphocyte-induced maturation protein 1,OTTHUMP00000016918,PR-domain zinc finger protein 1,PRDI-binding factor-1,beta-interferon gene positive-regulatory domain I binding factor,positive regulatory domain I-binding factor 1
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