MECOM polyclonal antibody
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More Files
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Western Blot (Cell lysate)
MECOM polyclonal antibody (Cat # PAB18553) (2 ug/mL) staining of A-549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MECOM.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human MECOM.
Sequence
C-DKESLHSTSH
Host
Goat
Theoretical MW (kDa)
170
Reactivity
Human
Specificity
This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
MECOM polyclonal antibody (Cat # PAB18553) (2 ug/mL) staining of A-549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MECOM
Entrez GeneID
2122Protein Accession#
NP_001098547.3;NP_005232.2;NP_004982.2;NP_001157471.1;NP_001157472.1Gene Name
MECOM
Gene Alias
EVI1, MDS1, PRDM3, MDS1-EVI1, AML1-EVI-1
Gene Description
MDS1 and EVI1 complex locus
Omim ID
165215Gene Ontology
HyperlinkOther Designations
AML1-EVI-1 fusion protein|oncogene EVI1
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Interactome
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Pathway
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Disease
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