ACO2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ACO2 polyclonal antibody (Cat # PAB18539) (0.01 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ACO2 polyclonal antibody (Cat # PAB18539) (3.8 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ACO2.
Immunogen
A synthetic peptide corresponding to internal region of human ACO2.
Sequence
C-QHVDVSPTSQRLQ
Host
Goat
Theoretical MW (kDa)
90
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.01-0.03 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ACO2 polyclonal antibody (Cat # PAB18539) (0.01 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ACO2 polyclonal antibody (Cat # PAB18539) (3.8 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACO2
Entrez GeneID
50Protein Accession#
NP_001089.1Gene Name
ACO2
Gene Alias
ACONM, MGC20605, MGC33908
Gene Description
aconitase 2, mitochondrial
Omim ID
100850Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq
Other Designations
OTTHUMP00000042146|OTTHUMP00000165920|aconitase 2|aconitate hydratase|citrate hydro-lyase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glyoxylate and dicarboxylate metabolism
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