S1PR1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HT-29 cells (Lane 1 and 4), LoVo cells (Lane 2) and A-549 cells (Lane 3), using S1PR1 polyclonal antibody (Cat # PAB18430).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of A-549 cells, using S1PR1 polyclonal antibody (Cat # PAB18430).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of S1PR1.
Immunogen
A synthetic peptide corresponding to residues surrounding T236 of human S1PR1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to S1PR1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HT-29 cells (Lane 1 and 4), LoVo cells (Lane 2) and A-549 cells (Lane 3), using S1PR1 polyclonal antibody (Cat # PAB18430).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using S1PR1 polyclonal antibody (Cat # PAB18430).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — S1PR1
Entrez GeneID
1901Protein Accession#
P21453Gene Name
S1PR1
Gene Alias
CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, FLJ58121, S1P1
Gene Description
sphingosine-1-phosphate receptor 1
Omim ID
601974Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq
Other Designations
G protein-coupled sphingolipid receptor|OTTHUMP00000012525|endothelial differentiation, sphingolipid G-protein-coupled receptor, 1|sphingosine 1-phosphate receptor EDG1
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Interactome
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Pathway
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Disease
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