HGS polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HUVEC cells (Lane 1), COLO cells (Lane 2) and 293 cells (Lane 3 and 4), using HGS polyclonal antibody (Cat # PAB18392).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HGS.
Immunogen
A synthetic peptide corresponding to residues surrounding Y334 of human HGS.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to HGS.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:100-1:500)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HUVEC cells (Lane 1), COLO cells (Lane 2) and 293 cells (Lane 3 and 4), using HGS polyclonal antibody (Cat # PAB18392).
Peptide "+" means "peptide blocking".Immunohistochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — HGS
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Interactome
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Pathway
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Disease
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Publication Reference
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Phosphoproteome analysis of HeLa cells using stable isotope labeling with amino acids in cell culture (SILAC).
Amanchy R, Kalume DE, Iwahori A, Zhong J, Pandey A.
Journal of Proteome Research 2005 Sep; 4(5):1661.
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The neurofibromatosis 2 tumor suppressor protein interacts with hepatocyte growth factor-regulated tyrosine kinase substrate.
Scoles DR, Huynh DP, Chen MS, Burke SP, Gutmann DH, Pulst SM.
Human Molecular Genetics 2000 Jul; 9(11):1567.
Application：IF, IP, WB, Human, STS26T cells.
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Phosphoproteome analysis of HeLa cells using stable isotope labeling with amino acids in cell culture (SILAC).
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