HDAC3 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HDAC3.
Immunogen
A synthetic peptide corresponding to residues surrounding S424 of human HDAC3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to HDAC3.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, using HDAC3 polyclonal antibody (Cat # PAB18361).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skin tissue, using HDAC3 polyclonal antibody (Cat # PAB18361).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with TNF-a (20 nM, 15 mins), using HDAC3 polyclonal antibody (Cat # PAB18361).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — HDAC3
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Interactome
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Disease
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Publication Reference
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Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein.
Gray SG, Iglesias AH, Lizcano F, Villanueva R, Camelo S, Jingu H, Teh BT, Koibuchi N, Chin WW, Kokkotou E, Dangond F.
The Journal of Biological Chemistry 2005 Aug; 280(31):28507.
Application:ChIP, WB, Human, Human mammalian cells.
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Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein.
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