KRT8 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with EGF (200 ng/mL, 30 mins), using KRT8 polyclonal antibody (Cat # PAB18332).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using KRT8 polyclonal antibody (Cat # PAB18332).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using KRT8 polyclonal antibody (Cat # PAB18332).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KRT8.
Immunogen
A synthetic peptide corresponding to residues surrounding S431 of human KRT8.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to KRT8.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with EGF (200 ng/mL, 30 mins), using KRT8 polyclonal antibody (Cat # PAB18332).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using KRT8 polyclonal antibody (Cat # PAB18332).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using KRT8 polyclonal antibody (Cat # PAB18332).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — KRT8
Entrez GeneID
3856Protein Accession#
P05787Gene Name
KRT8
Gene Alias
CARD2, CK8, CYK8, K2C8, K8, KO
Gene Description
keratin 8
Gene Ontology
HyperlinkGene Summary
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
Other Designations
cytokeratin 8|keratin, type II cytoskeletal 8
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Interactome
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Disease
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Publication Reference
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2A proteinase of human rhinovirus cleaves cytokeratin 8 in infected HeLa cells.
Seipelt J, Liebig HD, Sommergruber W, Gerner C, Kuechler E.
Journal of Biological Chemistry 2000 Jun; 275(26):20084.
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2A proteinase of human rhinovirus cleaves cytokeratin 8 in infected HeLa cells.
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