DDIT3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DDIT3.
Immunogen
A synthetic peptide corresponding to residues surrounding S30 of human DDIT3.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to DDIT3.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:50000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells (Lane 1), Jurkat cells (Lane 2) and HeLa cells (Lane 3 and 4), using DDIT3 polyclonal antibody (Cat # PAB18326).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using DDIT3 polyclonal antibody (Cat # PAB18326).
Peptide "+" means "peptide blocking".Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — DDIT3
Entrez GeneID
1649Protein Accession#
P35638Gene Name
DDIT3
Gene Alias
CEBPZ, CHOP, CHOP10, GADD153, MGC4154
Gene Description
DNA-damage-inducible transcript 3
Omim ID
126337Gene Ontology
HyperlinkOther Designations
C/EBP homologous protein|C/EBP zeta|CCAAT/enhancer-binding protein homologous protein|growth arrest- and DNA damage-inducible
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Interactome
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Pathway
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Disease
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Publication Reference
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Different induction of GRP78 and CHOP as a predictor of sensitivity to proteasome inhibitors in thyroid cancer cells.
Wang HQ, Du ZX, Zhang HY, Gao DX.
Endocrinology 2007 Jul; 148(7):3258.
Application:WB-Tr, Human, ARO, FRO cells.
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Different induction of GRP78 and CHOP as a predictor of sensitivity to proteasome inhibitors in thyroid cancer cells.
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