SOD1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SOD1.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human SOD1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Lyophilized
Purification
Immunoaffinity purification
Concentration
0.5 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(1 ug/mL)
Immunohistochemistry (Frozen sections)(1 ug/mL)
Immunocytochemistry (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis with SOD1 polyclonal antibody (Cat # PAB18299).
1 : Rat liver tissue lysate. 2 : Human placenta tissue lysate. 3 : MCF-7 whole cell lysate. 4 : CEM whole cell lysate. 5 : SW 620 whole cell lysate. 6 : COLO 320 whole cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemcal staining of HeLa cell with SOD1 polyclonal antibody (Cat # PAB18299). -
Gene Info — SOD1
Entrez GeneID
6647Gene Name
SOD1
Gene Alias
ALS, ALS1, IPOA, SOD, homodimer
Gene Description
superoxide dismutase 1, soluble
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq
Other Designations
Cu /Zn superoxide dismutase|Cu/Zn superoxide dismutase|SOD, soluble|indophenoloxidase A|superoxide dismutase, cystolic
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Interactome
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Pathway
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Disease
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