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Last updated: 2014/10/27
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GAPDH polyclonal antibody

  • Catalog # : PAB18293
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of GAPDH.
  • Immunogen:
  • A synthetic peptide corresponding to human GAPDH.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Lyophilized
  • Purification:
  • Immunoaffinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • Western Blot (1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(1 ug/mL)
    Immunohistochemistry (Frozen sections)(1 ug/mL)
    Immunocytochemistry (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis with GAPDH polyclonal antibody (Cat # PAB18293).
    1 : Rat heart tissue lysate. 2 : Rat brain tissue lysate. 3 : Rat liver tissue lysate. 4 : Rat skeletal muscle tissue lysate. 5 : MM453 whole cell lysate. 6 : MM231 whole cell lysate. 7 : SMMC whole cell lysate. 8 : HeLa whole cell lysate. 9 : HT1080 whole cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemcal staining of MCF-1 cell with GAPDH polyclonal antibody (Cat # PAB18293).
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Gene Information
  • Entrez GeneID:
  • 2597
  • Gene Name:
  • GAPDH
  • Gene Alias:
  • G3PD,GAPD,MGC88685
  • Gene Description:
  • glyceraldehyde-3-phosphate dehydrogenase
  • Gene Summary:
  • The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174431,OTTHUMP00000174432,aging-associated gene 9 protein,glyceraldehyde 3-phosphate dehydrogenase
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