ARAF polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ARAF.
Immunogen
A synthetic peptide corresponding to residues surrounding Y301/Y302 of human ARAF.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to ARAF.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with PMA (125 ng/mL, 30 mins), using ARAF polyclonal antibody (Cat # PAB18267).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ARAF polyclonal antibody (Cat # PAB18267).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ARAF
Entrez GeneID
369Protein Accession#
P10398Gene Name
ARAF
Gene Alias
A-RAF, ARAF1, PKS2, RAFA1
Gene Description
v-raf murine sarcoma 3611 viral oncogene homolog
Omim ID
311010Gene Ontology
HyperlinkOther Designations
OTTHUMP00000023212|Oncogene ARAF1|Ras-binding protein DA-Raf|v-raf murine sarcoma 3611 viral oncogene homolog 1
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Interactome
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Pathway
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Publication Reference
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Brain-derived neurotrophic factor-, epidermal growth factor-, or A-Raf-induced growth of HaCaT keratinocytes requires extracellular signal-regulated kinase.
Rossler OG, Thiel G.
American Journal of Physiology - Cell Physiology 2004 May; 286(5):C1118.
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Trihydrophobin 1 is a new negative regulator of A-Raf kinase.
Liu W, Shen X, Yang Y, Yin X, Xie J, Yan J, Jiang J, Liu W, Wang H, Sun M, Zheng Y, Gu J.
The Journal of Biological Chemistry 2004 Mar; 279(11):10167.
Application:WB-Tr, Human, Monkey, COS-1, HEK 293 cells.
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Brain-derived neurotrophic factor-, epidermal growth factor-, or A-Raf-induced growth of HaCaT keratinocytes requires extracellular signal-regulated kinase.
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