YWHAZ polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells, using YWHAZ polyclonal antibody (Cat # PAB18263).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using YWHAZ polyclonal antibody (Cat # PAB18263).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells, using YWHAZ polyclonal antibody (Cat # PAB18263).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of YWHAZ.
Immunogen
A synthetic peptide corresponding to residues surrounding T232 of human YWHAZ.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to YWHAZ.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells, using YWHAZ polyclonal antibody (Cat # PAB18263).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using YWHAZ polyclonal antibody (Cat # PAB18263).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells, using YWHAZ polyclonal antibody (Cat # PAB18263).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — YWHAZ
Entrez GeneID
7534Protein Accession#
P63104Gene Name
YWHAZ
Gene Alias
KCIP-1, MGC111427, MGC126532, MGC138156
Gene Description
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Omim ID
601288Gene Ontology
HyperlinkGene Summary
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq
Other Designations
14-3-3 protein/cytosolic phospholipase A2|14-3-3 zeta|OTTHUMP00000165851|OTTHUMP00000165852|OTTHUMP00000165854|OTTHUMP00000165858|OTTHUMP00000165859|OTTHUMP00000165860|phospholipase A2|protein kinase C inhibitor protein-1|tyrosine 3/tryptophan 5 -monooxyg
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Interactome
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Pathway
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Disease
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Publication Reference
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Isoform-specific differences in rapid nucleocytoplasmic shuttling cause distinct subcellular distributions of 14-3-3 sigma and 14-3-3 zeta.
van Hemert MJ, Niemantsverdriet M, Schmidt T, Backendorf C, Spaink HP.
Journal of Cell Science 2004 Mar; 117(Pt 8):1411.
Application：IF, WB, Human, HaCaT, Hela cells.
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Proteomic analysis reveals that 14-3-3sigma is down-regulated in human breast cancer cells.
Vercoutter-Edouart AS, Lemoine J, Le Bourhis X, Louis H, Boilly B, Nurcombe V, Revillion F, Peyrat JP, Hondermarck H.
Cancer Research 2001 Jan; 61(1):76.
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Isoform-specific differences in rapid nucleocytoplasmic shuttling cause distinct subcellular distributions of 14-3-3 sigma and 14-3-3 zeta.
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