TK1 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TK1.
Immunogen
A synthetic peptide corresponding to residues surrounding S13 human TK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to TK1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using TK1 polyclonal antibody (Cat # PAB18228).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using TK1 polyclonal antibody (Cat # PAB18228).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using TK1 polyclonal antibody (Cat # PAB18228).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — TK1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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[Effects of renal carcinoma cell line ACHN-derived exosomes on ACHN cell proliferation and apoptosis].
Yang L, Wu X, Luo C, He Y, Zhang Y, Chen X, Zhang L, Chen L.
Journal of Southern Medical University 2012 Oct; 32(10):1498.
Application:WB-Ce, Human, ACHN cells.
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The potential immunogenicity of the TK suicide gene does not prevent full clinical benefit associated with the use of TK-transduced donor lymphocytes in HSCT for hematologic malignancies.
Traversari C, Marktel S, Magnani Z, Mangia P, Russo V, Ciceri F, Bonini C, Bordignon C.
Blood 2007 Jun; 109(11):4708.
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An HSV-TK transgenic mouse model to evaluate elimination of fibroblasts for fibrosis therapy.
Tian B, Han L, Kleidon J, Henke C.
The American Journal of Pathology 2003 Aug; 163(2):789.
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Evaluation of HSV-tk gene therapy in a rat model of chemically induced hepatocellular carcinoma by intratumoral and intrahepatic artery routes.
Gerolami R, Cardoso J, Lewin M, Bralet MP, Sa Cunha A, Clément O, Bréchot C, Tran PL.
Cancer Research 2000 Feb; 60(4):993.
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[Effects of renal carcinoma cell line ACHN-derived exosomes on ACHN cell proliferation and apoptosis].
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