RPS6 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RPS6.
Immunogen
A synthetic peptide corresponding to residues surrounding S240 of human RPS6.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to RPS6.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with TNF-a (20 ng/mL, 2 mins), using RPS6 polyclonal antibody (Cat # PAB18219).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using RPS6 polyclonal antibody (Cat # PAB18219).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using RPS6 polyclonal antibody (Cat # PAB18219).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPS6
Entrez GeneID
6194Protein Accession#
P62753Gene Name
RPS6
Gene Alias
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Gene Description
ribosomal protein S6
Omim ID
180460Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
40S ribosomal protein S6|OTTHUMP00000021120|phosphoprotein NP33
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Interactome
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Pathway
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Publication Reference
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S6 kinase 2 potentiates interleukin-3-driven cell proliferation.
Cruz R, Hedden L, Boyer D, Kharas MG, Fruman DA, Lee-Fruman KK.
Journal of Leukocyte Biology 2005 Dec; 78(6):1378.
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S6 kinase 2 potentiates interleukin-3-driven cell proliferation.
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