MYB polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HUVEC cells, using MYB polyclonal antibody (Cat # PAB18191).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MYB polyclonal antibody (Cat # PAB18191).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using MYB polyclonal antibody (Cat # PAB18191).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MYB.
Immunogen
A synthetic peptide corresponding to residues surrounding S12 of human MYB.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to MYB.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HUVEC cells, using MYB polyclonal antibody (Cat # PAB18191).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MYB polyclonal antibody (Cat # PAB18191).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using MYB polyclonal antibody (Cat # PAB18191).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYB
Entrez GeneID
4602Protein Accession#
P10242Gene Name
MYB
Gene Alias
Cmyb, c-myb, c-myb_CDS, efg
Gene Description
v-myb myeloblastosis viral oncogene homolog (avian)
Omim ID
189990Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
Avian myeloblastosis viral (v-myb) oncogene homolog|OTTHUMP00000017258|c-myb protein (140 AA)|c-myb10A_CDS|c-myb13A_CDS|c-myb14A_CDS|c-myb8B_CDS|v-myb avian myeloblastosis viral oncogene homolog|v-myb myeloblastosis viral oncogene homolog
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Interactome
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Disease
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Publication Reference
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v-Myb mediates cooperation of a cell-specific enhancer with the mim-1 promoter.
Chayka O, Kintscher J, Braas D, Klempnauer KH.
Molecular and Cellular Biochemistry 2005 Jan; 25(1):499.
Application：ChIP, Chicken, BM2, HD11 cells.
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v-Myb mediates cooperation of a cell-specific enhancer with the mim-1 promoter.
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