Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
Immunogen:
A synthetic peptide corresponding to human EGFR.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Specificity:
This antibody is specific to EGFR.
Form:
Liquid
Purification:
Affinity purification
Concentration:
1 mg/mL
Recommend Usage:
Western Blot (1:500-1:1000) Immunohistochemistry (1:50-1:100) Immunofluorescence (1:500-1:1000) ELISA (1:10000) The optimal working dilution should be determined by the end user.
Western blot analysis of extracts from HUVEC cells, treated with Serum (20 %, 30 mins), using EGFR polyclonal antibody (Cat # PAB18169). Peptide "+" means "peptide blocking".
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR polyclonal antibody (Cat # PAB18169). Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using EGFR polyclonal antibody (Cat # PAB18169). Peptide "+" means "peptide blocking".
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations:
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor