BID polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells treated with H2O2 (100 uM, 30 mins) (lane1) and Jurkat cells treated with Etoposide (25 uM, 60 mins) (lane2), using BID polyclonal antibody (Cat # PAB18163).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using BID polyclonal antibody (Cat # PAB18163).
Peptide "+" means "peptide blocking".
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BID.
Immunogen
A synthetic peptide corresponding to human BID.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to BID.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells treated with H2O2 (100 uM, 30 mins) (lane1) and Jurkat cells treated with Etoposide (25 uM, 60 mins) (lane2), using BID polyclonal antibody (Cat # PAB18163).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using BID polyclonal antibody (Cat # PAB18163).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
-
Gene Info — BID
Entrez GeneID
637Protein Accession#
P55957Gene Name
BID
Gene Alias
FP497, MGC15319, MGC42355
Gene Description
BH3 interacting domain death agonist
Omim ID
601997Gene Ontology
HyperlinkGene Summary
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq
Other Designations
BH3-interacting domain death agonist|BID isoform ES(1b)|BID isoform L(2)|BID isoform Si6|Human BID coding sequence|OTTHUMP00000196197|apoptic death agonist|desmocollin type 4
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Three novel Bid proteins generated by alternative splicing of the human Bid gene.
Renshaw SA, Dempsey CE, Barnes FA, Bagstaff SM, Dower SK, Bingle CD, Whyte MK.
The Journal of Biological Chemistry 2004 Jan; 279(4):2846.
Application：WB, Human, HEK 293T, HeLa, Jurkat cells.
-
Three novel Bid proteins generated by alternative splicing of the human Bid gene.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com