EIF4EBP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01 U/mL, 15 mins), using EIF4EBP1 polyclonal antibody (Cat # PAB18142).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EIF4EBP1 polyclonal antibody (Cat # PAB18142).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF4EBP1.
Immunogen
A synthetic peptide corresponding to human EIF4EBP1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to EIF4EBP1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01 U/mL, 15 mins), using EIF4EBP1 polyclonal antibody (Cat # PAB18142).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EIF4EBP1 polyclonal antibody (Cat # PAB18142).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF4EBP1
Entrez GeneID
1978Protein Accession#
Q13541Gene Name
EIF4EBP1
Gene Alias
4E-BP1, 4EBP1, BP-1, MGC4316, PHAS-I
Gene Description
eukaryotic translation initiation factor 4E binding protein 1
Omim ID
602223Gene Ontology
HyperlinkGene Summary
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq
Other Designations
eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Phosphorylation of 4E-BP1 is mediated by the p38/MSK1 pathway in response to UVB irradiation.
Liu G, Zhang Y, Bode AM, Ma WY, Dong Z.
The Journal of Biological Chemistry 2002 Mar; 277(11):8810.
Application：IP, WB, Mouse, JB6 Cl 41 cells.
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Rapamycin-insensitive regulation of 4e-BP1 in regenerating rat liver.
Jiang YP, Ballou LM, Lin RZ.
The Journal of Biological Chemistry 2001 Apr; 276(14):10943.
Application：WB-Ti, Rat, Rat livers.
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Phosphorylation of 4E-BP1 is mediated by the p38/MSK1 pathway in response to UVB irradiation.
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