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Last updated: 2014/9/22
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CASP9 polyclonal antibody

  • Catalog # : PAB18116
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CASP9.
  • Immunogen:
  • A synthetic peptide corresponding to human CASP9.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to CASP9.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    Immunohistochemistry (1:50-1:100)
    Immunofluorescence (1:500-1:1000)
    ELISA (1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from NIH/3T3 cells treated with TNF-a (20 ng/mL, 30 min), using CASP9 polyclonal antibody (Cat # PAB18116).
    Peptide "+" means "peptide blocking".
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CASP9 polyclonal antibody (Cat # PAB18116).
    Peptide "+" means "peptide blocking".
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HUVEC cells, using CASP9 polyclonal antibody (Cat # PAB18116).
    Peptide "+" means "peptide blocking".
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 842
  • Gene Name:
  • CASP9
  • Gene Alias:
  • APAF-3,APAF3,CASPASE-9c,ICE-LAP6,MCH6
  • Gene Description:
  • caspase 9, apoptosis-related cysteine peptidase
  • Gene Summary:
  • This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq
  • Other Designations:
  • ICE-like apoptotic protease 6,OTTHUMP00000002322,OTTHUMP00000002323,OTTHUMP00000044594,apoptotic protease MCH-6,apoptotic protease activating factor 3,caspase 9,caspase 9, apoptosis-related cysteine protease
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