SMAD3 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SMAD3.
Immunogen
A synthetic peptide corresponding to human SMAD3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to SMAD3.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with UV (5 mins), Smad3 (Ab-425) antibody.SMAD3 polyclonal antibody (Cat # PAB18109).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SMAD3 polyclonal antibody (Cat # PAB18109).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMAD3
Entrez GeneID
4088Protein Accession#
P84022Gene Name
SMAD3
Gene Alias
DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396
Gene Description
SMAD family member 3
Omim ID
603109Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
Other Designations
MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|mad homolog JV15-2|mad protein homolog|mothers against decapentaplegic homolog 3
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Interactome
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Pathway
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Disease
- Alzheimer disease
- Anemia
- Asthma
- Bacteremia
- Breast cancer
- Breast Neoplasms
- Cleft Lip
- Cleft Palate
- Coronary Artery Disease
+ View More Disease
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Publication Reference
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Functional diversity of protein phosphatase-1, a cellular economizer and reset button.
Ceulemans H, Bollen M.
Physiological Reviews 2004 Jan; 84(1):1.
Application:IHC, WB, Human, Mammalian cells.
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TGF-beta down-regulates IL-6 signaling in intestinal epithelial cells: critical role of SMAD-2.
Walia B, Wang L, Merlin D, Sitaraman SV.
FASEB Journal 2003 Nov; 17(14):2130.
Application:WB, Dog, HT-29, MDCK cells.
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Smad and AML proteins synergistically confer transforming growth factor beta1 responsiveness to human germ-line IgA genes.
Pardali E, Xie XQ, Tsapogas P, Itoh S, Arvanitidis K, Heldin CH, ten Dijke P, Grundstrom T, Sideras P.
The Journal of Biological Chemistry 2000 Feb; 275(5):3552.
Application:Func, Human, DG75 cells.
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Functional diversity of protein phosphatase-1, a cellular economizer and reset button.
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