ACAN polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACAN.
Immunogen
A synthetic peptide corresponding to amino acids 336-369 of human ACAN.
Sequence
FFGVGGEED
Host
Rabbit
Reactivity
Human, Rat
Specificity
This antibody is specific to endogenous levels of fragment of activated ACAN resulting from cleavage adjacent to Asp369.
Form
Liquid
Recommend Usage
Western Blot (1:500~1:1000)
Immunohistochemistry (1:50~1:100)
ELISA(1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25 uM, 60 mins), using ACAN polyclonal antibody (Cat # PAB18067).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ACAN polyclonal antibody (Cat # PAB18067).
Peptide "+" means "with peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — ACAN
Entrez GeneID
176Protein Accession#
P16112Gene Name
ACAN
Gene Alias
AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK
Gene Description
aggrecan
Gene Ontology
HyperlinkGene Summary
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq
Other Designations
aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122)|aggrecan proteoglycan|cartilage-specific proteoglycan core protein|chondroitin sulfate proteoglycan 1|large aggregating proteo
-
Interactome
-
Disease
-
Publication Reference
-
Aggrecan glycoforms contribute to the molecular heterogeneity of perineuronal nets.
Matthews RT, Kelly GM, Zerillo CA, Gray G, Tiemeyer M, Hockfield S.
Journal of Neuroscience 2002 Sep; 22(17):7536.
-
SOX9 enhances aggrecan gene promoter/enhancer activity and is up-regulated by retinoic acid in a cartilage-derived cell line, TC6.
Sekiya I, Tsuji K, Koopman P, Watanabe H, Yamada Y, Shinomiya K, Nifuji A, Noda M.
The Journal of Biological Chemistry 2000 Apr; 275(15):10738.
-
Overexpression of a single helix-loop-helix-type transcription factor, scleraxis, enhances aggrecan gene expression in osteoblastic osteosarcoma ROS17/2.8 cells.
Liu Y, Watanabe H, Nifuji A, Yamada Y, Olson EN, Noda M.
The Journal of Biological Chemistry 1997 Nov; 272(47):29880.
-
Aggrecan glycoforms contribute to the molecular heterogeneity of perineuronal nets.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com