ACTN2/ACTN3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACTN2/ACTN3.
Immunogen
A synthetic peptide corresponding to N-terminus of human ACTN2/ACTN3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total ACTN2/ACTN3 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells (Lane 1), HepG2 cells (Lane 2) and HUVEC cells (Lane 3 and lane 4), using ACTN2/ACTN3 polyclonal antibody (Cat # PAB17362).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ACTN2/ACTN3 polyclonal antibody (Cat # PAB17362).
Peptide "+" means "with peptide blocking". -
Gene Info — ACTN2
Entrez GeneID
88Protein Accession#
P35609 (Gene ID : 88);Q08043 (Gene ID : 89)Gene Name
ACTN2
Gene Alias
CMD1AA
Gene Description
actinin, alpha 2
Omim ID
102573Gene Ontology
HyperlinkGene Summary
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq
Other Designations
F-actin cross-linking protein|OTTHUMP00000037963|alpha-actinin skeletal muscle
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Gene Info — ACTN3
Entrez GeneID
89Protein Accession#
P35609 (Gene ID : 88);Q08043 (Gene ID : 89)Gene Name
ACTN3
Gene Alias
MGC117002, MGC117005
Gene Description
actinin, alpha 3
Omim ID
102574Gene Ontology
HyperlinkGene Summary
Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments [provided by RefSeq
Other Designations
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Interactome
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Pathway
- Adherens junction
- Adherens junction
- Arrhythmogenic right ventricular cardiomyopathy (ARVC)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC)
- Focal adhesion
- Focal adhesion
- Leukocyte transendothelial migration
- Leukocyte transendothelial migration
- Regulation of actin cytoskeleton
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Disease
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Publication Reference
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Fine mapping and genomic structure of ACTN2, the human gene coding for the sarcomeric isoform of alpha-actinin-2, expressed in skeletal and cardiac muscle.
Tiso N, Majetti M, Stanchi F, Rampazzo A, Zimbello R, Nava A, Danieli GA.
Biochemical and Biophysical Research Communications 1999 Nov; 265(1):256.
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ZASP: a new Z-band alternatively spliced PDZ-motif protein.
Faulkner G, Pallavicini A, Formentin E, Comelli A, Ievolella C, Trevisan S, Bortoletto G, Scannapieco P, Salamon M, Mouly V, Valle G, Lanfranchi G.
The Journal of Cell Biology 1999 Jul; 146(2):465.
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Cloning and characterization of two human skeletal muscle alpha-actinin genes located on chromosomes 1 and 11.
Beggs AH, Byers TJ, Knoll JH, Boyce FM, Bruns GA, Kunkel LM.
The Journal of Biological Chemistry 1992 May; 267(13):9281.
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Fine mapping and genomic structure of ACTN2, the human gene coding for the sarcomeric isoform of alpha-actinin-2, expressed in skeletal and cardiac muscle.
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