EEF2 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells treated with serum (10%, 30 mins), using EEF2 polyclonal antibody (Cat # PAB17280).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EEF2 polyclonal antibody (Cat # PAB17280).
Peptide "+" means "with peptide blocking".
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EEF2.
Immunogen
A synthetic peptide corresponding to residues surrounding threonine 56 of human EEF2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total EEF2 protein.
Form
Liquid
Recommend Usage
Western Blot (1:300-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells treated with serum (10%, 30 mins), using EEF2 polyclonal antibody (Cat # PAB17280).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EEF2 polyclonal antibody (Cat # PAB17280).
Peptide "+" means "with peptide blocking". -
Gene Info — EEF2
Entrez GeneID
1938Protein Accession#
P13639Gene Name
EEF2
Gene Alias
EEF-2, EF2
Gene Description
eukaryotic translation elongation factor 2
Omim ID
130610Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq
Other Designations
polypeptidyl-tRNA translocase
-
Interactome
-
Disease
-
Publication Reference
-
Exercise rapidly increases eukaryotic elongation factor 2 phosphorylation in skeletal muscle of men.
Rose AJ, Broholm C, Kiillerich K, Finn SG, Proud CG, Rider MH, Richter EA, Kiens B.
The Journal of Physiology 2005 Oct; 569(Pt 1):223.
-
Levels of mTOR and its downstream targets 4E-BP1, eEF2, and eEF2 kinase in relationships with tau in Alzheimer's disease brain.
Li X, Alafuzoff I, Soininen H, Winblad B, Pei JJ.
The FEBS Journal 2005 Aug; 272(16):4211.
-
Exercise rapidly increases eukaryotic elongation factor 2 phosphorylation in skeletal muscle of men.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com