FOXO3 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FOXO3.
Immunogen
A synthetic peptide corresponding to residues surrounding S253 of human FOXO3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total FOXO3 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells treated with using Serum.
Lane 1 : Using FOXO3 polyclonal antibody (Cat # PAB16986).
Lane 2 : Using the same antibody preincubated with synthesized peptide.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOXO3 polyclonal antibody (Cat # PAB16986).Enzyme-linked Immunoabsorbent Assay
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Gene Info — FOXO3
Entrez GeneID
2309Protein Accession#
O43524Gene Name
FOXO3
Gene Alias
AF6q21, DKFZp781A0677, FKHRL1, FKHRL1P2, FOXO2, FOXO3A, MGC12739, MGC31925
Gene Description
forkhead box O3
Omim ID
602681Gene Ontology
HyperlinkGene Summary
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000016944|forkhead box O3A|forkhead homolog (rhabdomyosarcoma) like 1|forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Resveratrol preconditioning increases methionine sulfoxide reductases A expression and enhances resistance of human neuroblastoma cells to neurotoxins.
Wu PF, Xie N, Zhang JJ, Guan XL, Zhou J, Long LH, Li YL, Xiong QJ, Zeng JH, Wang F, Chen JG.
The Journal of Nutritional Biochemistry 2013 Jun; 24(6):1070.
Application:IF, WB-Ce, Human, SH-SY5Y cells.
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Down-regulation of manganese-superoxide dismutase through phosphorylation of FOXO3a by Akt in explanted vascular smooth muscle cells from old rats.
Li M, Chiu JF, Mossman BT, Fukagawa NK.
The Journal of Biological Chemistry 2006 Dec; 281(52):40429.
Application:EMSA, IF, WB-Ce, WB-Tr, Rat, Vascular smooth muscle cells.
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Direct transcriptional regulation of Bim by FoxO3a mediates STI571-induced apoptosis in Bcr-Abl-expressing cells.
Essafi A, Fernandez de Mattos S, Hassen YA, Soeiro I, Mufti GJ, Thomas NS, Medema RH, Lam EW.
Oncogene 2005 Mar; 24(14):2317.
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Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell proliferation.
Seoane J, Le HV, Shen L, Anderson SA, Massague J.
Cell 2004 Apr; 117(2):211.
Application:IF, IP, WB-Tr, Human, Mouse, HaCaT, U87MG cells, Mouse embryos.
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Resveratrol preconditioning increases methionine sulfoxide reductases A expression and enhances resistance of human neuroblastoma cells to neurotoxins.
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