IL6 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using IL6 polyclonal antibody (Cat # PAB16919) shows detection of a band ~24 KDa in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown. After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1 : 500 dilution. Detection occurred using HRP conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer and FemtoMax™ Chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant IL6.
Immunogen
Recombinant protein corresponding to full length human IL6.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Recommend Usage
ELISA (1:10000)
Western Blot (1:300-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot using IL6 polyclonal antibody (Cat # PAB16919) shows detection of a band ~24 KDa in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown. After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1 : 500 dilution. Detection occurred using HRP conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer and FemtoMax™ Chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).Enzyme-linked Immunoabsorbent Assay
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Gene Info — IL6
Entrez GeneID
3569Gene Name
IL6
Gene Alias
BSF2, HGF, HSF, IFNB2, IL-6
Gene Description
interleukin 6 (interferon, beta 2)
Gene Ontology
HyperlinkGene Summary
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq
Other Designations
interleukin 6
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression of TGF-beta isoforms by Thy-1+ and Thy-1- pulmonary fibroblast subsets: evidence for TGF-beta as a regulator of IL-1-dependent stimulation of IL-6.
Silvera MR, Sempowski GD, Phipps RP.
Lymphokine Cytokine Res 1994 Oct; 13(5):277.
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Enhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble IL-6 receptor from urine and reversion by monoclonal antibody.
Novick D, Shulman LM, Chen L, Revel M.
Cytokine 1992 Jan; 4(1):6.
Application：Blocking, ELISA, Func, Human, Mouse, Mouse plasmacytoma cells, T-47D cells.
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Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity.
Novick D, Eshhar Z, Revel M, Mory Y.
Hybridoma 1989 Oct; 8(5):561.
Application：WB-Ce, Recombinant protein.
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Expression of TGF-beta isoforms by Thy-1+ and Thy-1- pulmonary fibroblast subsets: evidence for TGF-beta as a regulator of IL-1-dependent stimulation of IL-6.
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