NOS1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of rat brain tissue lysate (10 mg/lane). Blot was probed with NOS1 polyclonal antibody (Cat # PAB15602) at 1 : 250 (Lane 1) or 1 : 1000 (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin fixed, citric acid treated parafin sections of adult rat striatum. Sections were probed with NOS1 polyclonal antibody (Cat # PAB15602) then anti-Rabbit HRP before detection using DAB.
Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant NOS1.
Immunogen
Recombinant protein corresponding to C-terminus of human NOS1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is conserved in human and rat eNOS, and has low homology to other NOS family members.
Form
Liquid
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of rat brain tissue lysate (10 mg/lane). Blot was probed with NOS1 polyclonal antibody (Cat # PAB15602) at 1 : 250 (Lane 1) or 1 : 1000 (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin fixed, citric acid treated parafin sections of adult rat striatum. Sections were probed with NOS1 polyclonal antibody (Cat # PAB15602) then anti-Rabbit HRP before detection using DAB.
Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOS1
Entrez GeneID
4842Gene Name
NOS1
Gene Alias
IHPS1, NOS, nNOS
Gene Description
nitric oxide synthase 1 (neuronal)
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. This gene encodes a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in this gene are associated with infantile hypertrophic pyloric stenosis type 1
Other Designations
neuronal nitric oxide synthase
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Interactome
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