PSMB8 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PSMB8 polyclonal antibody (Cat # PAB14398) (0.01 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PSMB8.
Immunogen
A synthetic peptide corresponding to human PSMB8.
Sequence
C-DVSDLLHQYREANQ
Host
Goat
Theoretical MW (kDa)
29.8, 30.4
Reactivity
Human
Specificity
Approx 22 KDa band observed in human liver, lung and lymph node lysates (calculated MW of 29.8 KDa according to precursor NP_004150.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schwarz et al, J Immunol. 2000 Jul 15;165(2):768-78. PMID: 10878350.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PSMB8 polyclonal antibody (Cat # PAB14398) (0.01 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PSMB8
Entrez GeneID
5696Protein Accession#
NP_004150.1;NP_683720.2Gene Name
PSMB8
Gene Alias
D6S216, D6S216E, LMP7, MGC1491, PSMB5i, RING10, beta5i
Gene Description
proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Omim ID
177046Gene Ontology
HyperlinkGene Summary
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq
Other Designations
OTTHUMP00000029420|OTTHUMP00000029421|OTTHUMP00000062981|low molecular weight protein 7|macropain subunit C13|multicatalytic endopeptidase complex subunit C13|protease component C13|proteasome (prosome, macropain) subunit beta type 8 (large multifunctiona
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Interactome
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Pathway
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Disease
- Acute Disease
- Alveolitis
- Arthritis
- Asthma
- Bronchiolitis
- Brucellosis
- Cardiovascular Diseases
- Coronary Disease
- Dermatitis
+ View More Disease
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Publication Reference
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Variation in HLA class I antigen-processing genes and susceptibility to human papillomavirus type 16-associated cervical cancer.
Deshpande A, Wheeler CM, Hunt WC, Peyton CL, White PS, Valdez YE, Nolan JP.
The Journal of Infectious Diseases 2008 Feb; 197(3):371.
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Variation in HLA class I antigen-processing genes and susceptibility to human papillomavirus type 16-associated cervical cancer.
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